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Creating a streamlinedmanual fluorescent stainingmethod for neuroscience
Blekinge Institute of Technology, Faculty of Engineering, Department of Mechanical Engineering.
2021 (English)Independent thesis Basic level (university diploma), 12 credits / 18 HE creditsStudent thesisAlternative title
Creating a streamlinedmanual fluorescent stainingmethod for neuroscience (English)
Abstract [en]

This thesis will conduct an attempt of simplifying the currently used stainingmethod of the protein profiling group at The Karolinska Institute led by Jan Mulder. By utilizing iterative product development several prototypes were created to first fit the criteria’s specified and secondly best retain and evenly spread a solution across the tissue sections. A realization made early on was that to be able to create the correct spreading of such a small volume of a solution one must make use of the capillary effect created in narrow spaces. From numerous of iterations of design and testing a product that outperformed the rest was found. That product was the prototype Quadlane leaf drain. The product needs to be stored in specific climates to ensure proper functionality during staining. To fulfil that need a storage system based on inserts were invented that can be placed inside of a contained area with saturated air to ensure sufficient hydration. 

Abstract [sv]

Denna avhandling kommer att göra ett försök att förenkla den för närvarande använda färgningsmetoden för proteinprofileringsgruppen vid Karolinska Institutet ledd av Jan Mulder. Genom att använda sig utav Iterativproduktutveckling kunde flera prototyper skapas för att uppfylla kriterierna specificerade och för att bäst behålla och jämnt sprida vätska över vävnadssnitten. En tidig insikt var att för att kunna skapa en korrekt spridningav en så liten volym vätska måste kapilläreffekten som skapas i trånga utrymmen utnyttjas. Efter flertalet iterationer av design och test av olika prototyper visade sig en lösning vara bättre. Denna lösning var prototypen Quadlane leaf drain. Utöver den framtagna produkten behövdes även ett ställe att förvara produkten vid inkubationer. Detta görs med ett framtaget förvarings system som är uppbyggt av insatser som seden kan bli placerade i ett förslutet område med mättad luft för att försäkra ett väl hydrerat system. 

Place, publisher, year, edition, pages
2021. , p. 84
Keywords [en]
Streamlined, Product development, Prototyping, Immunofluorescencestaining, 3D-printing, Innovation
Keywords [sv]
Streamlined, Produktutveckling, Prototyping, Immunoflourescens-färgning, 3D-printing, Innovation.
National Category
Production Engineering, Human Work Science and Ergonomics
Identifiers
URN: urn:nbn:se:bth-21659OAI: oai:DiVA.org:bth-21659DiVA, id: diva2:1566949
External cooperation
Karolinska Insitutet
Subject / course
MT1475 Thesis
Educational program
MTGMI Bachelor of Science in Mechanical Engineering
Supervisors
Examiners
Available from: 2021-06-18 Created: 2021-06-15 Last updated: 2021-06-18Bibliographically approved

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fulltext(2789 kB)194 downloads
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