Change search
ReferencesLink to record
Permanent link

Direct link
Protamine reduces whole blood platelet aggregation after cardiopulmonary bypass
Blekinge Institute of Technology, Faculty of Health Sciences, Department of Health.
Show others and affiliations
2015 (English)In: Scandinavian Cardiovascular Journal, ISSN 1401-7431, E-ISSN 1651-2006, Vol. 50, no 1, 58-63 p.Article in journal (Refereed) Published
Abstract [en]

Objectives: Platelet dysfunction is an important cause of postoperative bleeding after cardiac surgery. Protamine is routinely used for reversal of heparin after cardiopulmonary bypass (CBP), but may affect platelet aggregation. We assessed changes in platelet function in relation to protamine administration. Design: Platelet aggregation was analyzed by impedance aggregometry before and after protamine administration in 25 adult cardiac surgery patients. Aggregation was also studied after in vitro addition of heparin and protamine. The activators adenosine diphosphate (ADP), thrombin receptor activating peptide-6 (TRAP), arachidonic acid (AA) and collagen (COL) were used. Results: Platelet aggregation was reduced by approximately 50% after in vivo protamine administration; ADP 640 ± 230 (AU*min, mean ± SD) to 250 ± 160, TRAP 939 ± 293 to 472 ± 260, AA 307 ± 238 to 159 ± 143 and COL 1022 ± 350 to 506 ± 238 (all p < 0.001). Aggregation was also reduced after in vitro addition of protamine alone with activators ADP from 518 ± 173 to 384 ± 157 AU*min p < 0.001, and AA 449 ± 311 to 340 ± 285 (p < 0.01) and protamine combined with heparin (1:1 ratio) with activators ADP to 349 ± 160 and AA to 308 ± 260 (both p < 0.001); and COL from 586 ± 180 to 455 ± 172 (p < 0.05). Conclusions: Protamine given after CPB markedly reduces platelet aggregation. Protamine added in vitro also reduces platelet aggregation, by itself or in combination with heparin. © 2015 Taylor & Francis

Place, publisher, year, edition, pages
Taylor & Francis, 2015. Vol. 50, no 1, 58-63 p.
Keyword [en]
cardiopulmonary bypass; platelet aggregation; platelet function tests; protamine
National Category
Cardiac and Cardiovascular Systems
URN: urn:nbn:se:bth-11011DOI: 10.3109/14017431.2015.1099720ISI: 000365693900009ScopusID: 2-s2.0-84944936680OAI: diva2:873592
Available from: 2015-11-24 Created: 2015-11-24 Last updated: 2016-02-03Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Berglund, Johan
By organisation
Department of Health
In the same journal
Scandinavian Cardiovascular Journal
Cardiac and Cardiovascular Systems

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 49 hits
ReferencesLink to record
Permanent link

Direct link