A start-up faces many challenges during different phases of its journey to become a successful sustainable business. A successful scaling of the business is critical to the potential of the start-up and its ability to generate revenue and grow. Leadership has a very important impact on a start-up, navigating the business through different phases and their challenges. Even though it is recognized that leadership is impacted by many factors such as team members and internal and external factors around the organization and evolves with the time to handle those challenges, little is known about the impact of leadership on startups during the scaling phase, particularly when dealing with challenges such as human and financial capital shortage. This research is designed to explore leadership effect on startup’s scaling up.The study was conducted through interviewing successful founders and leaders how have navigated the new venture through scaling up phase, the thematic analysis shows that diverse influences of leadership qualities and traits on startup scaling aspects challenges human and financial capital. There is a dynamic interplay between leadership characteristics and organizational contexts in the successful scaling of startups. Leadership qualities such as educational background, and prior leadership experience are scrutinized for their influence on strategic decision-making and team management during critical growth phases. The study highlights how leadership styles evolve from transactional to transformational to meet the increasing complexity of scaling enterprises. Additionally, team composition and their attributes and organizational changes, including hiring practices and structural adjustments, are pivotal in accommodating the evolving demands of the business, reflecting a thorough integration of leader attributes and environmental factors in scaling success.The study of leadership characteristics, team members, and organizational context in startup scaling provides several promising avenues for future research such as study in leadership evolution within specific industries or comparative studies across industries, global and cultural variations affecting leadership, role of gender and diversity in leadership, the effectiveness of different leadership styles and many more.